A photosynthesis microfluidic chamber comprises: at least one communicating space a plurality of microfluidic channels respectively connected with the at least one communicating space at least one saline water injection microfluidic channel respectively connected to at least one communicating space a plurality of filtering corks respectively connected to the plurality of microfluidic channels and to at least one saline water injection microfluidic channel at the other end and a light source irradiating the at least one communicating space, the plurality of microfluidic channels and the at least one saline water injection microfluidic channel, wherein chloroplast and saline water are injected into at least one communicating space, the plurality of microfluidic channels and the at least one saline water injection microfluidic channel.一種光合作用微流道室,包括:至少一連通空間;複數微流道,分別連接至該至少一連通空間;至少一食鹽水注入微流道,分別連接至該至少一連通空間;複數過濾栓,分別連接至複數微流道與至少一食鹽水注入微流道的另一端;以及一光源,照射至少一連通空間、複數微流道與至少一食鹽水注入微流道,其中,葉綠體與生理食鹽水被注入至少一連通空間、複數微流道與至少一食鹽水注入微流道。100‧‧‧光合作用微流道室101‧‧‧連通空間102‧‧‧微流道103‧‧‧食鹽水注入微流道104‧‧‧過濾栓105‧‧‧光源106‧‧‧綠葉體