The present invention relates to a vaccine against Vibrio infection in shrimps and a method for preparing the same. In particular, the vaccine of the invention comprises a mixture of heat-inactivated Vibrio cells and formalin-inactivated Vibrio cells, which induce enhanced and prolonged immunity against Vibrio infection in shrimp.本發明係關於一種對抗蝦子之弧菌感染之疫苗及其製備方法。具體而言,本發明之該疫苗包含熱滅活弧菌細胞與甲醛溶液滅活弧菌細胞的混合物,其誘導蝦類增強及延長的對抗弧菌感染之免疫性。