BEECH, Robert,BEGTRUP, Gavi,HEIKENFELD, Jason,BERTRAND, Jacob, A.
申请号：
EP17840149
公开号：
EP3496596A4
申请日:
2017.08.08
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
The disclosed invention includes integrative electrochemical aptamer-based sensors for use in wearable biofluid sensing devices. The disclosed integrative EAB sensors are configured to detect very low concentrations of target analytes in a sweat or biofluid sample by aggregating signals from individual sensing elements over time until a signal threshold is reached. Signal aggregation is accomplished through various retention structures that extend the time sensing elements retain target analyte molecules. Embodiments include attaching complementary primers and functional groups to the aptamer, covering such retention structures with blockers until analyte capture, or coating the sensor electrode with a hydrophilic and hydrophobic monolayer. The invention also includes methods of using the disclosed integrative sensors. Some embodiments of the disclosed method include tracking time to signal threshold to estimate analyte concentration.
