The present invention provides a transgenic rodent, wherein the genome of said rodent comprising the mutant of NGF-² gene, said rodent expressing the mutein of NGF-². The present invention also provides the method for obtaining the transgenic rodent, and the methods of preparing the corresponding mutein of mutant NGF-² gene, and the protein obtained using this method. The present invention provides a new way to produce human NGF, and exploit a new way to investigate the function of Mutant NGF-² and its receptor in the level of whole animal, and to screen and purify the mutant NGF-² of high activity and safety.
