PROBLEM TO BE SOLVED: To provide a technique of preparing differentiated cells without the contamination with undifferentiated stem cells, in differentiated cells induced from stem cells and to provide a pharmaceutical composition containing a stem cell survival inhibitor for inhibiting in vivo neoplastic transformation of a cell pharmaceutical composition containing differentiated cells derived from stem cells.SOLUTION: The invention provides a culture medium for differentiated cells derived from stem cells by which differentiated cells without contamination with undifferentiated stem cells can be obtained by compounding, into the undifferentiated stem cells, atrovastatin which has a specific survival inhibiting activity and inhibits the survival of undifferentiated stem cells without inducing the apoptosis of the differentiated cells.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT【課題】幹細胞から誘導される分化細胞において、未分化な幹細胞の混入のない分化細胞を調製する技術と、幹細胞由来の分化細胞を含む細胞医薬組成物の、生体内での腫瘍化を抑制するための幹細胞生存抑制剤を含む医薬組成物の提供。【解決手段】未分化な幹細胞に特異的な生存抑制活性を有し、分化細胞の細胞死を誘発することなく、未分化幹細胞の生存を抑制するアトルバスタチンを含むことで、未分化幹細胞の混入がない分化細胞を得ることができる、幹細胞由来の分化細胞用培地。【選択図】図1
