Luca Gattinoni;Enrico Lugli;Mario Roederer;Nicholas P. Restifo
发明人:
Luca Gattinoni,Enrico Lugli,Mario Roederer,Nicholas P. Restifo
申请号：
US16410327
公开号：
US20190264175A1
申请日:
2019.05.13
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating naïve T cells from a mammal, wherein the mammal is not a mouse； b) activating the naïve T cells and expanding the numbers of naïve T cells in the presence of one or more non-specific T cell stimuli, one or more cytokines, and a GSK-3beta inhibitor. Also provided are methods of producing an isolated T memory stem cell population, the method comprising a) isolating lymphocytes from a mammal； b) sorting the lymphocytes using flow cytometry into a population comprising a phenotype comprising i) CD95+, CD45RO−；, and CCR7+； and ii) CD62L+ or one or more of CD27+, CD28+, CD45RA+, and CD127+ to produce an isolated T memory stem cell population. Further embodiments of the invention provide related cells, populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer.
