A method for preventing and/or treating myocardial injury and its related diseases of a subject, comprising administering an effective amount of plasminogen for preventing and/or treating to a subject who is in risk of suffering from myocardial injury, is suspected to suffer from myocardial injury, or suffers from myocardial injury. A drug, a pharmaceutical composition, a product, and a kit comprising plasminogen and used for preventing and/or treating myocardial injury and its related diseases of a subject.