A single use container (10, 40, 70) for holding a plurality of endoscope valves during a cleaning and disinfection procedure defines a plurality of chambers (34, 64, 84) which are isolated from each other and configured to receive a single valve such that a valve in one chamber cannot contact a valve in another chamber. A plurality of apertures (36, 58, 86) in each chamber allow fluid flow therethrough. A closure device (14, 44, 92) is operable to close each chamber (34, 64, 84). An attachment device (38, 68, 77) is configured for attaching the container (10, 40, 70) to an endoscope and to prevent detachment without breaking of the attachment device (38, 68, 77).