The invention relates to a method and a device for separating blood using a centrifuge in order to obtain different blood fractions. A separation container may be filled with blood via a feed connection that can be aseptically connected to the container, the relevant blood fraction may be removed from the separation container via a removal connection that can be aseptically connected to the container and both the feed device and the removal device folln a respective closed system during the sterile connection to the separation container. The separation container may have at least one feed connection that can be aseptically connected to the container and at least one removal connection that can be aseptically connected to the container, to which hermetically sealed feed and removal devices can be aseptically attached in a sterile manner.
