An imaging device includes a visible light source 22, an excitation light source 23 that irradiates an excitation light to excite indocyanine green administered to a patient; an evaluation light source 24 that irradiates the light having the wavelength corresponding to the fluorescence from indocyanine green; a aperture mechanism 60 that narrows only visible light between visible light and fluorescence; a camera 21 capable of detecting fluorescence and visible light; a focal adjustment mechanism 70 that executes focal adjustment of the camera 21 using the light irradiated from the evaluation light source 24.