KANG, KYUNG SUN,강경선,SEO, MIN SOO,서민수,PARK, SANG BUM,박상범
申请号：
KR1020110091746
公开号：
KR1020130028294A
申请日:
2011.09.09
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: Separation and characterization of watton jelly-originated mesenchyme stem cell of canine are provided to have capability of differentiating cultured cell into ectoderm, mesoderm, or endoderm originated cell by culturing stem cell separated from watton jelly of canine and animals.CONSTITUTION: As for the separation and characterization of watton jelly-originated mesenchyme stem cell of canine, stem cell separated from the watton jelly of canine and animal is cultured. The cultivated cell is collected. The characteristics of preparation method of multipotent stem cell of watton jelly of canine and animal are as follows. The immunological characteristics of negative are shown to CD3, CD11c, CD28, CD34, CD38, CD45, CD41a, and CD62L as a human marker, and the immunological characteristics of positive are shown to CD90 and CD105 as a human marker. The cell has the capability of differentiating into ectoderm, mesoderm, or endoderm originated cells. The cell has the cultivation capability of undivided condition with passage vaccination of 14 or more. The canine and animal are selected from dog, cornice lupus, coyote, jackal, and fox.COPYRIGHT KIPO 2013본 발명은 개과 동물의 와튼 젤리 유래 다분화능 줄기세포의 제조방법, 상기 방법으로 제조된 다분화능 줄기세포, 상기 다분화능 줄기세포를 분화시키는 방법 및 상기 다분화능 줄기세포 또는 이로부터 분화된 세포를 유효성분으로 포함하는 세포 치료제에 관한 것이다. 본 발명의 개과 동물의 와튼 젤리 유래 다분화능 줄기세포는 개의 조직에서 유래되어, 연골세포, 골세포, 지방세포, 신경세포 등의 중배엽 유래 세포로 용이하게 분화될 수 있으므로, 애완동물관련 시장에서 개의 치료에 널리 활용될 수 있을 것이다.
