The present invention relates to use of a combination of a histone deacetylase inhibitor and a protein kinase inhibitor for the manufacture of a medicament for treating or preventing a tumor. The present invention further relates to a pharmaceutical composition comprising a method comprising a histone deacetylase inhibitor and a protein kinase inhibitor as active ingredients, and a method of treating or preventing a cancer by combined use of a histone deacetylase inhibitor and a protein kinase inhibitor.本發明涉及組蛋白去乙醯化酶抑制劑與蛋白激酶抑制劑之組合在製備用於治療或預防腫瘤的藥物中的用途,本發明還涉及包含組蛋白去乙醯化酶抑制劑和蛋白激酶抑制劑作為活性成分的藥物組合物以及聯用組蛋白去乙醯化酶抑制劑和蛋白激酶抑制劑治療或預防癌症的方法。