VOGELS RONALD,FRIESEN ROBERT HEINZ EDWARD,MEIJBERG JAN WILEM,IMPAGLIAZZO ANTONIETTA,ALARD PHILIPPE,LOVERIX STEFAN,RADO&ScaronEVI&Cacute KATARINA
申请号：
NZ62597312
公开号：
NZ625973A
申请日:
2012.11.27
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Discloses an influenza hemagglutinin stem domain polypeptide comprising (a) an H1 HA1 N-terminal segment comprising the amino acids 1-52, covalently linked by a linking sequence of 0-10 amino acid residues to an H1 5 HA1 C-terminal stem segment comprising the amino acids 321-end of HAl, and (b) an influenza hemagglutinin HA2 domain, wherein the C-terminal amino acid residue of the HA1 C-terminal stem segment is glutamine (Q), serine (S), threonine (T), asparagine (N), aspartic acid (D) or glutamic acid (E), and wherein one or more of the amino acids on position 406, 409, 413 and 416 have been changed into an amino acid selected from the group consisting of S,T,N,Q,R,H,K,D,E and G, and wherein the polypeptides comprise a disulphide bridge between the amino acids of position 324 and 436 wherein the polypeptide comprises hemagglutinin stem domains from HA of the influenza virus A/ Brisbane/59/2007(SEQ ID NO:1) or A/California/04/2009 (SEQ ID NO: 159), and wherein the numbering is based on the numbering of amino acids of the H1N1 influenza strain A/Brisbane/59/2007(SEQ ID NO:1) and an influenza hemagglutinin stem domain polypeptide comprising (a) an H3 HA1 N-terminal segment comprising the amino acids 1-61, covalently linked by a linking sequence of 0-10 amino acid residues to an H3 HA1 C-terminal stem segment comprising the amino acids 323-end of HA1, and (b) an influenza hemagglutinin HA2 domain wherein the C-terminal amino acid residue of the HA1 C-terminal stem segment is glutamine (Q), serine (S), threonine (T), asparagine (N), aspartic acid (D) or glutamic acid (E) and wherein one or more of the amino acids on position 40l, 408, 411, 415 and 418 have been changed into an amino acid selected from the group consisting of S,T,N,Q,R,H,K,D,E and G, and wherein the polypeptides comprise a disulphide bridge between the amino acids of position 326 and 438 wherein the polypeptide comprises HA domains of A/Wisconsin/67/2005 (SEQ ID NO: 89) or A/Hong Kong/1/1968 (SEQ ID NO:
