Biosurfactant produced by yeast are widely used in various fields such as biomedicine, agriculture, food and cosmetic industry. Biosurfactant production process and culture conditions is already known however, the used of expensive culture medium components in the production of biosurfactant like sophorolipids (SL) from yeast is not an assurance for better production yield at the end of culture period. In addition, biosurfactant from the yeast like Candida bombicola is known of having generally regarded as safe (GRAS) status and also gaining positive attention because of its biodegradability and as a non-toxic substitute to chemical surfactant. The present invention relates to liquid culture medium added with dried mango peel powder as culture component of Candida bombicola BCRC22302 to produce sophorolipid (SL) biosurfactant.