To provide an image pickup unit that can prevent axis deviation of a movable lens barrel and obtain high-quality picked-up image, a stopper member is positioned and fixed in a state in which the stopper member is abutted against an outer circumferential surface of a rear group lens barrel, movement of a movable lens barrel to a retraction side along a photographing optical axis is restricted by contact of a stopper section of the stopper member and an operation rod, and a movable lens is held in a position for realizing a second focal length.