HERBAL COMPOSITION FOR TREATMENT OF MASTITIS AND PROCESS OF PREPARATION THEREOF The present disclosure provides a herbal composition comprising plant extract of Cucurbita maxima and plant extract of Annona Squamosa. The present disclosure also provides a process of preparation of herbal composition for treatment or prevention of mastitis comprising: washing a plant part of Cucurbita Maxima and Annona Squamosa followed by drying to remove excess moisture to obtain dried plant part; powdering and sieving the dried plant part through a mesh to obtain powder; adding a solvent to the powder to obtain a solution; extracting the extract from the solution to obtain a mixture; stirring the mixture to obtain a herbal composition.The herbal composition of the present disclosure is effective in the treatment of mastitis in live stock.