A ligation device, an unlocking method, and a ligation instrument. The ligation device (100) is characterized in that: the ligation device comprises a clip (110) and a tightening tube (120); a distal end of the clip (110) is used for ligation; the tightening tube (120) is provided with an accommodating channel (125); a near end of the clip (110) is butt-jointed with the accommodating channel (125); the near end of the clip (110) can enter and exit the tightening tube (120); the clip (110) is provided with a latch member (112); the tightening tube (120) is provided with a latch site (122); and the latch member (112) is matched with the latch site (122). When the latch member (112) is locked to the latch site (122), the latch member (112) is operated so that the latch member (112) is unlocked from the latch site (122). Or the clip (110) is further provided with an unlocking member (140) butt-jointed with the latch member (112); the unlocking member (140) is operated so that the latch member (112) is unlocked from the latch site (122). The ligation device (100) can be re-opened after ligation and locking, and has no secondary damage to a ligated tissue (400).L'invention concerne un dispositif de ligature, un procédé de déverrouillage et un instrument de ligature. Le dispositif de ligature (100) est caractérisé en ce que : le dispositif de ligature comprend une attache (110) et un tube de serrage (120) ; une extrémité distale de l'attache (110) est utilisée pour la ligature ; le tube de serrage (120) est pourvu d'un canal de réception (125) ; une extrémité proche de l'attache (110) est assemblée bout à bout avec le canal de réception (125) ; l'extrémité proche de l'attache (110) peut entrer et sortir du tube de serrage (120) ; l'attache (110) est pourvue d'un élément de verrouillage (112) ; le tube de serrage (120) est pourvu d'un site de verrouillage (122) ; et l'élément de verrouillage (112) est mis en correspondance avec le site de verrouillage (122). Lorsque l'éléme