Disclosed herein is the use of the Ganoderic acids for the preparation of a medicament for preventing a subject from developing heart failure or injury. The Ganoderic acids is a mixture of Ganoderic acid A, B, C, C5, C6, D, E, G and ganoderenic acid D.在此揭示一種靈芝酸混合物的新穎用途,用來製造一種可防止心臟壞死或損傷之藥劑。所述靈芝酸混合物,包含靈芝酸A、B、C、C5、C6、D、E、G及靈芝烯酸D(ganoderenic acid D)之成分。