Chinese Academy of Sciences;Institute of Genetics and Developmental Biology
发明人:
Gao, Caixia,Li, Jun
申请号：
AU2016309392
公开号：
AU2016309392A1
申请日:
2016.08.15
申请国别(地区):
AU
年份:
2018
代理人:
摘要：
A method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution is provided, which comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I), and substituting proline (P) at position 12 with serine (S) to obtain a plant， i.e., a glyphosate-resistant plant. A method capable of generating a site-directed nucleotide substitution and a fragment substitution utilizing a CRISPR-mediated NHEJ pathway to substitute a region between two gRNA sites by designing the two gRNA sites is provided.
