To provide a method for cryopreservation of umbilical cord tissue that allows a satisfactory number of living cells to be recovered after thawing.SOLUTION: The present invention provides a method for cryopreservation of umbilical cord tissue, including an immersion step for immersing umbilical cord tissue containing living cells in a cryopreservation solution containing a cryoprotective agent and glucose, and a freezing step for freezing the umbilical cord tissue in a state where it is immersed in the cryopreservation solution.SELECTED DRAWING: None【課題】解凍後に回収される生細胞数が良好な、臍帯組織の凍結保存方法を提供する。【解決手段】本発明に係る凍結保存方法は、臍帯組織を凍結保存する方法であって、凍害防御剤およびグルコースを含む凍結保存用溶液に、生細胞を含む臍帯組織を浸漬する浸漬工程と、上記臍帯組織を、上記凍結保存用溶液に浸漬した状態で凍結する凍結工程と、を含む。【選択図】なし