FIELD: medicine.SUBSTANCE: group of inventions relates to medicine and medical equipment, namely to devices and methods of calibration for fluorescent image formation. Fluorescence standard for identification of changes in illumination during image formation performed in multitude of time moments, contains fluorescent object which fluoresces in response to the light with wavelength in approximate range from 375 nm to 430 nm. Fluorescent object has three areas with different fluorescent response: the first layer of the material, which has fluorescent response to the light with wavelength in approximate range from 375 nm to 430 nm the second layer of the material, which weakens fluorescent response of the first said layer, and the third layer, which additionally weakens fluorescence of the first layer beyond the limits of weakening characteristic of the second layer. The second layer only partially covers the first layer and the third layer of the material partially covers the second layer. Said standard of fluorescence is used to realise the method of identification of changes in illumination during image formation by means of a camera. For this purpose fluorescent object is placed in front of the camera. After that the first image of fluorescent object is captured by means of the light with wavelength in approximate range from 375 nm to 430 nm, the second image of fluorescent image is captured by means of the light with wavelength in the same range. After that, obtained fluorescent responses of fluorescent object on the first and second images of.EFFECT: creation of device and method of calibration for fluorescent image formation.19 cl, 4 dwgГруппа изобретений относится к медицине и медицинской технике, а именно к устройствам и способам калибровки для флуоресцентного формирования изображений. Эталон флуоресценции для идентификации изменений освещения во время формирования изображений, выполняемого во множестве моментов времени, содержит флуоресцентный объект, которы