An image sensor includes: a plurality of pixels arranged in a two-dimensional matrix form and configured to receive light from outside and generate and output an imaging signal depending on an amount of the received light a transfer unit configured to transfer the imaging signal output from each of the pixels a sample-and-hold unit configured to hold and output the imaging signal transferred from the transfer unit a reference voltage generation unit configured to generate and output a reference voltage an amplifier connected to the sample-and-hold unit and to the reference voltage generation unit and configured to amplify and output the imaging signal input from the sample-and-hold unit using the reference voltage as a reference and a control unit configured to control the reference voltage to be input to the amplifier during at least one of a horizontal blanking period and a video signal period of the imaging signal.