A method for the filling of containers with autologous fresh blood components, comprising the following phases of : a) providing an equipment (1) defining a transit channel (3) having at least a first gap (3a) associated with an empty syringe (4) or the like, at least a second gap (3b) associated with a bag (5) or the like containing an autologous fresh blood component and at least a third gap (3c) connected to a plurality of containers (6) to be filled b) insulating the second gap (3b) from the third gap (3c) and placing in communication the first gap (3 a) with the third gap (3c) c) suctioning the air contained in the containers (6) to be filled by means of said syringe (4) so as to define a vacuum inside them d) insulating the first gap (3a) from the third gap (3c) and placing in communication the latter with the second gap (3b), the containers suctioning the contents of the bag (5) due to the effect of the vacuum defined inside them!