KOTIN, ROBERT MICHAEL,ALKAN, OZAN,JONES, ANNALIESE,KERR, DOUGLAS ANTHONY,MALAKIAN, ARA KARL,SIMMONS, MATTHEW JOHN,WRIGHT, TERESA L.
申请号：
CA3075168
公开号：
CA3075168A1
申请日:
2018.09.07
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
CeDNA vectors having linear and continuous structure can be produced in high yields and used for effective transfer and expression of a transgene. ceDNA vectors comprise an expression cassette and two different ITR sequences derived from AAV genomes in a specified order. Some ceDNA vectors provided herein further comprise cis-regulatory elements and provide high gene expression efficiencies. Further provided herein are methods and cell lines for reliable and efficient production of the linear, continuous and capsid-free DNA vectors.
