Method for forming antimicrobial complex surface, being performed during processes of an anodic treatment including the following steps being worked on a work piece: pretreatment, oxidation of anodic treatment, acid pickling, staining and pole sealing, at least comprising the following steps: providing an argentum containing solution introducing the argentum solution during the processes of the anodic treatment and providing argentum particle based on the argentum solution as a argentum particle source, so as to form an antimicrobial complex surface on the work piece.一種形成抗菌複合表面的加工方法,其在一陽極製程的過程裡執行,其中該陽極製程依序包含對一工件進行如下步驟:前處理步驟、陽極氧化步驟、酸洗步驟、染色步驟及封孔步驟,所述抗菌複合表面的加工方法至少包括如下步驟:提供一含銀溶液;於進行該陽極製程的過程裡,加入該含銀溶液;以及藉由該含銀溶液作為銀粒子來源以提供數個銀粒子,並使該工件的外部含有所述銀粒子,從而在該工件的外部形成該抗菌複合表面。本發明更依據上述加工方法提供一種抗菌複合表面,以有效抑制微生物在經陽極氧化處理的金屬表面上的生長。