METHODS AND COMPOSITIONS FOR LARGE-SCALE ISOLATION OF VERY SMALL EMBRYONIC-LIKE (VSEL) STEM CELLS

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专利权人:

Janina Ratajczak

发明人:

Janina Ratajczak,Mariusz Z. Ratajczak

申请号：

US14008796

公开号：

US20140154219A1

申请日:

2012.04.02

申请国别(地区):

US

年份:

2014

代理人:

摘要：

Methods for purifying very small embryonic-like (VSEL) stem cells from populations of cells suspected of including VSEL stem cells are provided. In some embodiments, the methods include (a) providing a population of cells suspected of including a VSEL stem cell and (b) isolating a CD45neg/GlyAneg/CD133+/ALDHhigh subpopulation, a CD45neg/GiyAneg/CD133+/ALDlow subpopulation, a CD45neg/Linneg/SSEA-4+/ALDHhigh subpopulation, a CD45neg/Linneg/SSEA-4/AiOlow subpopulation, or any combination thereof from the population, whereby a VSEL stem cell is purified from the population. Also provided are methods for generating in vitro hematopoietic colonies derived from VSEL stem cells and methods for generating lympho-hematopoietic chimerism in a subject.

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