FEDERALNOE GOSUDARSTVENNOE BYUDZHETNOE OBRAZOVATELNOE UCHREZHDENIE VYSSHEGO OBRAZOVANIYA "KRASNOYARSKIJ GOSUDARSTVENNYJ MEDITSINSKIJ UNIVERSITET IMENI PROFESSORA V.F. VOJNO-YASENETSKOGO MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIJSKOJ FEDERATSII"
发明人:
Semenov Eduard Vasilevich,Slepov Evgenij Vladimirovich,Zukov Ruslan Aleksandrovich
申请号：
RU20170105638
公开号：
RU2670655(C2)
申请日:
2017.02.20
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to oncology, and can be used to determine the risk of recurrence of superficial bladder cancer after surgical treatment. For this purpose, the first morning urine sample of a patient with bladder cancer T1N0M0, TaN0M0 or TisN0M0. Portion was centrifuged at 2,000 rpm for 20 min to precipitate the cell elements. After this, the supernatant was drained, 2 ml of PBS was added to the pellet. Then, resuspension was carried out by pipetting for 1–2 min and centrifuged at 2,000 rpm for 10 min. This procedure for washing the cell pellet was carried out twice. Supernatant was then decanted, 0.5 ml of PBS was added to the pellet. Resulting cell suspension was then resuspended for 1–2 minutes. To this, monoclonal antibodies to CD13 labeled with phycoerythrin in a volume of 20 mcl were added. Stirred on a personal vortex for 20 seconds. Monoclonal antibodies to CD45 labeled with fluorescein with isothiocyanate in a volume of 20 mcl were added. Again mi
