The present invention provides the composition of the bio-ink comprising of gelatin, sodium alginate, collagen peptide (Hydrolysed Type I Collagen), secondary mouse muscle myoblast C2C12 cells, and complete growth medium (89% - Dulbecco’s Modified Eagle Medium (DMEM), 10% - Fetal bovine serum and 1% penicillin-streptomycin). Gelatin and sodium alginate are naturally occurring biodegradable biopolymers. Gelatin is thermo-reversibly polymerizable hydrogel and alginate has cross-linking properties which makes the combination of the two, a suitable scaffold for rapid prototyping of constructs having live cells. Addition of CaCl2 provides Ca2+ ions for cross-linking sodium alginate which strengthens the structure of the scaffold thereby providing stability. The bio-ink forms a suitable scaffold with adequate mechanical strength, and also provides an efficient material for growth and differentiation of muscle cells.
