A portable gas transfer device for point-of-use sterilization at a sterilization site includes: a housing； a pressurized gas canister held by the housing； a first passageway in fluid communication with the pressurized gas canister and configured to supply pressurized pre-sterilization gas from the pressurized gas canister to the site； a gas discharge canister held by the housing； and a second passageway in fluid communication with the gas discharge canister and configured to supply post-sterilization gas from the site to the gas discharge canister. Additional related devices, systems and methods are provided.
