The present invention relates to a transgenic cloned canine overexpressing phosphoenol pyruvate carboxykinase (PEPCK), and a production method thereof. More specifically, the present invention relates to a nuclear transferred egg of a canine formed by transferring the nucleus of a somatic cell derived from a canine transformed with a PEPCK gene to an enucleated egg, a production method thereof, a transgenic cloned canine overexpressing PEPCK produced by transferring the nuclear transferred egg into the oviduct of a surrogate mother, and a production method thereof. The transgenic cloned canine overexpressing PEPCK gene according to the present invention, as an animal model of diabetes mellitus, can be used for investigating the genetic cause of type 2 diabetes mellitus and developing a diagnostic reagent and a therapeutic agent.La présente invention a pour objet un canidé transgénique cloné surexprimant la phosphoénol pyruvate carboxykinase (PEPCK), et son procédé de production. Plus spécifiquement, la présente invention concerne un œuf à transfert nucléaire dun canidé formé par le transfert du noyau dune cellule somatique issue dun canidé transformé au moyen dun gène PEPCK en un œuf énucléé, son procédé de production, un canidé transgénique cloné surexprimant PEPCK produit par le transfert de lœuf ayant subi un transfert nucléaire dans loviducte dune mère porteuse, et son procédé de production. Le canidé transgénique cloné surexprimant le gène PEPCK selon la présente invention, en tant que modèle animal du diabète sucré, peut être utilisé pour la recherche de la cause génétique du diabète sucré de type 2 et le développement dun réactif diagnostique et dun agent thérapeutique.본 발명은 PEPCK (Phosphoenol Pyruvate Carboxykinase)를 과발현하는 형질전환 복제 개과 동물 및 이의 생산방법에 관한 것이다. 보다 구체적으로 본 발명은 PEPCK 유전자로 형질전환된 개과 동물 유래 체세포 핵을 탈핵된 난자에 이식하여 형성된 개과 동물의 핵 이식란과 이의 생산방법 및 상기 핵 이식란을 대리모의 난관에 이식하여 생산된 PEPCK를 과발현하는 형질전환 복제 개과 동물 및 이의 생산방법에 관한 것이다. 본 발명에 따른 PEPCK 유전자를 과발현하는 형질전환 복제 개과 동물은 당뇨
