A decontamination operation chamber 30 is partitioned into a first operation chamber 31 and a second operation chamber 32. A connecting portion that connects the first operation chamber and the second operation chamber can be closed. The pressure of the first operation chamber 31 and the second operation chamber 32 is adjusted by a pressure-adjusting mechanism. When the connecting portion is opened and the first operation chamber 31 and the second operation chamber 32 are connected, an inlet portion for the first operation chamber 31 and an outlet portion for the second operation chamber 32 are closed and air flow is generated from the second operation chamber 32 to the first operation chamber 31 by the pressure-adjusting mechanism. When carrying an object A into an aseptic manipulation chamber 10, the object A is housed in the first operation chamber 31 and a first decontamination operation is performed, then the object A is transferred to the second operation chamber 32 and a second decontamination operation is performed.
