An apparatus and a method for non-invasive blood glucose monitoring and a method for analyzing biological molecule are provided. The apparatus for non-invasive blood glucose monitoring includes a light source for generating light, a beam splitter leading the light into an eyeball and focuses on the eyeball, and a set of photo detectors for measuring optical rotatory distribution (ORD) information and absorption energy information of the light, and a processing unit. The processing unit receives and processes the ORD information and the absorption energy information to obtain an ORD difference and an absorption energy difference between the light emitted from the light source and the light transmitted to the set of photo detectors, and analyzes the ORD difference and the absorption energy difference to obtain a glucose information, and since the glucose information has a corresponding relationship with a blood glucose information, the blood glucose information may be read.
