Cloning, yeast expression, purification and biological activity of the extension region of the soybean 7S globulin alfa' subunit involved in HEP G2 cell cholesterol homeostasis
Paolo Morazzoni,Antonella Riva,Cesare Ponzone,Davide Berlanda,Marcello Duranti,Alessandro Consonni
申请号：
US13378605
公开号：
US08569455B2
申请日:
2010.06.16
申请国别(地区):
US
年份:
2013
代理人:
摘要：
A truncated form of α′ chain (eα′), the soybean 7S globulin, active in controlling the cholesterol and triglyceride homeostasis in in vitro and in vivo models, was cloned and expressed in the yeast Pichia pastoris. The recombinant polypeptide spanned 142 amino acid residues from the N-terminal side and included the N-terminal extension region of the soybean alpha′ subunit. The eα′ polypeptide was purified by conventional biochemical techniques and its potential to modulate the activity of the LDL-receptor was evaluated in a human hepatoma cell line (Hep G2) by monitoring the uptake and degradation of labeled LDL.
