Elizabeth M. Simpson,Wyeth W. Wasserman,Robert A. Holt,Steven J. Jones,Daniel Goldowitz,Elodie Portales-Casamar,Cletus DSouza,Vikramjit Chopra
申请号：
US12538771
公开号：
US08383803B2
申请日:
2009.08.10
申请国别(地区):
US
年份:
2013
代理人:
摘要：
Isolated polynucleotides comprising a PITX3 promoter are provided, where a PITX3 regulatory element is operably joined to a PITX3 basal promoter utilizing a non-native spacing between the promoter and regulatory elements. The promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, etc.
