BATHE, BRIGITTE,KREUTZER, CAROLINE,THIERBACH, GEORG
申请号：
PL07821449
公开号：
PL2078085T3
申请日:
2007.10.17
申请国别(地区):
PL
年份:
2015
代理人:
摘要：
Coryneform bacterium mutant comprises a gene coding for a 2-methylcitrate dehydratase polypeptide (I) with a proteinogenic amino acid other than L-proline at position 272. Independent claims are also included for: (1) coryneform bacterium mutant produced by treating a coryneform bacterium with a mutagenic agent, isolating and multiplying the resulting mutants, preparing nucleic acids from the mutants, producing a nucleic acid molecule from the nucleic acids by PCR using a primer comprising at least 15 consecutive nucleotides from the sequence between positions 1 and 1563 of SEQ ID NO:3 or SEQ ID NO:7 (not given in the specification) and a primer comprising at least 15 consecutive nucleotides from the complementary sequence between positions 3000 and 1567 of SEQ ID NO:3 or SEQ ID NO:7, sequencing the nucleic acid molecule and determining the encoded amino acid sequence, optionally comparing the sequence with SEQ ID NO:6 (not given in the specification), and identifying a mutant containing a polynucleotide coding for a polypeptide with a proteinogenic amino acid other than L-proline at position 272； (2) isolated polynucleotide (II) coding for (I)； (3) isolated polynucleotide coding for an amino acid sequence corresponding to positions 19-57 of SEQ ID NO:2 (not given in the specification) with a proteinogenic amino acid other than L-proline at position 272； (4) producing a recombinant coryneform bacterium by transferring (II) into a coryneform bacterium so that the bacterium's 2-methylcitrate dehydratase gene is replaced by (II) and multiplying the resulting bacterium； (5) producing a recombinant microorganism by transferring (II) into a microorganism, replicating (II) in the microorganism and multiplying the resulting microorganism； (6) recombinant microorganism containing (II)； (7) vector containing (II)； (8) recombinant microorganism containing the vector； (9) producing a L-amino acid by culturing a coryneform bacterium mutant as above so that the L-amino acid is en
