A medical observation apparatus includes: an image sensor configured to image an observation target and generate a medical captured image by sequential reading； and a controller configured to control lighting of light sources configured to irradiate light to the observation target. The controller is configured to perform a first control including a control of lighting a light source configured to irradiate white light, and a second control including a control of lighting a light source configured to irradiate excitation light, and the first control is performed in a period that spans over a boundary of successive frames.
