FRENDEWEY, DAVID,DROGUETT, GUSTAVO,GAGLIARDI, ANTHONY,KUNO, JUNKO,AUERBACH, WOJTEK,VALENZUELA, DAVID M.
申请号：
CA2953559
公开号：
CA2953559A1
申请日:
2015.06.26
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein； and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.
