Disclosed is an antibody or fragment thereof that specifically binds to a human RON protein, wherein said antibody or fragment thereof comprises a CDRH1 having a sequence GFTFSSYLMT (SEQ ID NO: 29), a CDRH2 having a sequence NIKQDGSEKYYVDSVKG (SEQ ID NO: 31), a CDRH3 having a sequence DGYSSGRHYGMDV (SEQ ID NO: 33), a CDRL1 having a sequence RASQSVSRYLA (SEQ ID NO: 35), a CDRL2 having a sequence DASNRAT (SEQ ID NO: 37) and a CDRL3 having a sequence QQRSNWPRT (SEQ ID NO: 39). Also disclosed is a use of the antibody or fragment thereof for the manufacture of a medicament for inhibiting angiogenesis, tumor growth, proliferation of tumor cells, migration of tumor cells, invasion of tumor cells, RON activation, or phosphorylation of RON, MAPK and/or Akt.
