PARK, SANG KI,박상기,SUH, YEONG JUN,서영준,PARK, SANG KIKR,SUH, YEONG JUNKR
申请号：
KR1020140026766
公开号：
KR1020150104866A
申请日:
2014.03.06
申请国别(地区):
KR
年份:
2015
代理人:
摘要：
The present invention relates to a recombinant nucleic acid molecule for controlling mitochondrial activities using light stimulation and, more specifically, to a recombinant nucleic acid molecule in which a gene encoding ABCB10 protein and a gene encoding rhodopsin protein are linked to each other and a recombinant vector containing the same, a composition for controlling mitochondrial activities, or a method for controlling mitochondrial activities using light. In case of using the recombinant nucleic acid molecule according to the present invention, the recombinant nucleic acid molecule in which a gene encoding ABCB10 protein and a gene encoding rhodopsin protein are linked to each other is transfected into a cell. Thus, the function or activity of rhodopsin protein can be controlled by applying light stimulation. As a result, mitochondrial activities can be reversibly controlled by light stimulation with spatiotemporal precision. In addition, since light is used as a stimulus, light can be controlled with precise in various ranges by regulating the wavelength and intensity of a light source such as LED, laser, or the like. Moreover, as optic technology is developed, the advantages can be continually applied. Thus, the method is simple and has an excellent effect in controlling mitochondrial activities. Therefore, the roles of mitochondria with various functions such as a signal transduction process, energy production, and the like in each tissue can be deeply understood, so the present invention can be importantly applied to diagnose and study diseases related to mitochondrial activities.본 발명은 광 자극을 이용하여 미토콘드리아 활성을 조절하기 위한 재조합 핵산 분자에 관한 것으로서, 보다 구체적으로는 ABCB10 단백질을 암호화하는 유전자와 로돕신 단백질을 암호화하는 유전자를 연결시킨 재조합 핵산 분자 및 이를 포함하는 재조합 벡터, 미토콘드리아 활성 조절용 조성물, 또는 빛을 이용한 미토콘드리아 활성 조절 방법에 관한 것이다.본 발명에 따른 재조합 핵산 분자를 이용하면, ABCB10 단백질을 암호화하는 유전자와 로돕신 단백질을 암호화하는 유전자가 연결된 재조합 핵산 분자가 세포로 transfection됨으로써, 광 자극의 인가에 따라 로돕신 단백질의 기능 또는 활성을 조절할 수 있어, 그 결과 시간적, 공간적으로 섬세하게 빛 자극으로 미토콘드리아의
