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CELLULES SOUCHES ADULTES ADIPEUSES HUMAINES PLURIPOTENTES : ISOLEMENT, CARACTÉRISATION ET IMPLICATIONS CLINIQUES
专利权人:
The Regents of the University of California
发明人:
申请号:
EP14801169.5
公开号:
EP2999780B1
申请日:
2014.05.22
申请国别(地区):
EP
年份:
2019
代理人:
摘要:
Methods for the efficient isolation and use of pluripotent adipose-derived stem cells (PASCs) are provided. In certain embodiments the methods involve providing an adipose tissue sample from which the stromal vascular fraction is co-cultured with the adipocyte fraction. PASCs can be isolated with a high degree of purification without requiring an additional cell enrichment process (e.g. cell sorting). PASCs and their conditioned media can be used for tissue regeneration within hours of harvesting the adipose tissue, and without requiring cell expansion. PASCs can grow as floating individual cells, as clusters of cells, or attached to surface(s) of the culture vessel. PASCs do not produce teratomas in vivo, nor do they induce immunorejection upon transplantation, and they achieve a high efficiency in grafting. The cells and compositions can be used for cell therapy and to screen new drugs.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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