A biological artificial blood vessel and the preparation method for producing the biological artificial blood vessel. The biological artificial blood vessel is composed of a substrate 1 made of an animal blood vessel, and a coating 2 bound on the inner surface of the substrate 1. The animal blood vessel is immobilized by crosslinking through an immobilizing agent and processed to remove antigens. The coating 2 contains anti-coagulation components. The preparation method for the biological artificial vessels comprises the steps of collecting blood vessels from animals as the substrate 1, performing processing, removing fat, immobilizing, eliminating antigens, and anti-coagulation modification to the substrate 1.
