An inter-dental cleaning brush comprises a primary floss member that is constituted by a one-half diameter tooth cleaning filament； at least two brush filaments, each of which is connected perpendicularly to the primary floss member, and bundle of brush filaments are spaced by a fixed interval, a section of the primary floss member to which the brush filaments are connected is twisted about an axial direction to form a helical section； a secondary floss member, which is constituted by a one-half diameter tooth cleaning filament and is jointed to the helical section to have the helical section constrained without reversely rotating to restore.一種牙間隙清潔刷具包含一主牙線，係由二分之一直徑之潔牙線構成；至少二毛刷線，各該毛刷線與該主牙線垂直接合，且各束毛刷線之間具有等間距離，將該主牙線上接合有該毛刷線之線段經軸向旋轉後於其上形成一螺旋區段；一副牙線，係由二分之一直徑之潔牙線構成，其與該螺旋區段接合，俾使該螺旋區段受到限制而不會逆向旋轉回復。
