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In vitro method for reprogramming or increasing the life-span of recipient cells and uses of the recipient cells
专利权人:
ADVANCED CELL TECHNOLOGY INC.
发明人:
申请号:
IL14717901
公开号:
IL147179A
申请日:
2001.12.19
申请国别(地区):
IL
年份:
2015
代理人:
摘要:
Methods for de-differentiating or altering the life-span of desired “recipient” cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and/or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods.
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