David Rabuka,Gregory W. deHart,Patrick Holder,Jeanne Baker
申请号:
US14975403
公开号:
US09951367B2
申请日:
2015.12.18
申请国别(地区):
US
年份:
2018
代理人:
摘要:
The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.