The purpose of the notification is to isolate and initiate in vitro culture the microspore using the early-middle to medium-spring barley stage, which is that freshly cut logs cut into vines. One cm fragments and placed in a cooled blender chamber. The coils are blended twice into 0,4 M mannitol at idle speed. Blinded logs are passed through a 100m sterile filter into a container on ice. The remaining logs are collected from the filter and blended twice into 0,4 M mannitol to release as many microspores as possible and re-filtered pera 100m filter. The micro-spore suspension is transferred to the test tube and rotates at 120-130 x g through 8-10 mines at 4-oC temperature and the micro-spore residue is then removed in 0,55 M maltose. The suspension is transferred to a new sterile test tube and 0,4 M mannitol is applied to the surface and rotates in a concentration gradient at 100-110 x g through 8-10 min at 4,C temperature to separate viable microsporeson the edge of the phases, from the non-viable in the pellet. The micropores at the edge of the phase in the ring are collected and rotated to be deposited at 120-130 x g through 8-10-min at 4-oC temperature. The microspore sediment is then dried, and the microspores are suspended in the SMB1 medium to the density 100 000 -150 000 microspore per 1 ml of the medium on the shelf and incubated favourably by 48 h at temperature 25 oC. Then the SMB1 medium is replaced by KBP modified by adding the hydrolysis of casein and organic acids to the density 100 000-15000 microspore per 1 ml of nutrients on a shelf. In vitro culture on a modified KBP medium is conducted for seven days at temperature 25oC in the dark. After this time, fresh food is added to GDP and culture continues for two weeks under the same conditions as the shake-up at 100 rpm. The KBP medium is then removedand the discarded multi-cellular structures are transferred to a medium that differentiates KBPD into biblical filters. Culture takes place at 25oC temperat
