Provided is a system for screening anti-tumour substances. The system comprises a first expression vector which encodes a first regulatory factor and a second expression vector which encodes a biological macromolecule, and the expression of the biological macromolecule can be co-regulated by the first regulatory factor and an inducer. Also provided are a method for preparing the system, a method for screening anti-tumor substances by using the system and anti-tumour substances obtained by screening.
