The present invention relates to be used to prepare slurries, the slurries include cell factor and coagulation factor, and prepare the composition of fibrin gel, what wherein this method provided isolated sample includes blood platelet and/orleukocytes to different different gravity, to obtain the cell factor and coagulation factor that supernatant has high concentration. Derived products can be obtained by the method the invention also includes serum and other include them. Disclose using product, particularly its be used as regeneration.