Recombinant North American Type 1 Swine Producing and Respiratory Syndrome Virus and Methods of Use

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专利权人:

SOUTH DAKOTA STATE UNIVERSITY

发明人:

FANG, YING,HENNINGS, JANE

申请号：

DK08771834

公开号：

DK2181189T3

申请日:

2008.06.24

申请国别(地区):

DK

年份:

2012

代理人:

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.

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