Systems and methods for adjusting for non-invasive measurement of biological analytes. Exemplary methods include: illuminating an analyte using first light, the first light having a first excitation wavelength； detecting a first spectrum from the analyte illuminated by the first light, the first spectrum including a first Raman signal and fluorescence； illuminating the analyte using second light, the second light having a second excitation wavelength； detecting a second spectrum； illuminating the analyte using third light, the third light having a third excitation wavelength； detecting a third spectrum； recovering the first Raman signal using the first spectrum, the second spectrum, and the third spectrum using an inverse transform； and using the first Raman signal to identify and measure at least one molecule of the analyte using a database of identified Raman signals.
