MA YIFAN,ZHOU XIANGJUN,CHEN SHANG,CAI LINTAO,WANG CE,LIU PENG
申请号：
IN201627043671
公开号：
IN201627043671A
申请日:
2016.12.21
申请国别(地区):
IN
年份:
2017
代理人:
摘要：
The present invention provides a method for preparing dendritic cell loaded with antigen the method comprising the steps of adding serum free cell culture medium containing granulocyte macrophage colony stimulating factor (GM CSF) and interleukin (IL) 4 into mononuclear cells culturing in an incubator at 37 under 5% carbon dioxide for 5 days adding target antigen wrapped cationic liposome and culturing for 8 24 hours to obtain target antigen loaded dendritic cell.
